碱性成纤维细胞生长因子对兔全脑缺再灌注后血清炎症因子的影响
原作者： 马岳峰 张 茂 江观玉 徐善祥 干建新 发布日期：2003-07-29

【摘要】 目的 观察碱性成纤维细胞生长因子（bFGF）对全脑缺血再灌注后血清炎症因子的影响，探讨其脑保护作用的可能机制。 方法 24只大白兔随机均分为假手术组（A组）、对照组（B组）和bFGF组（C组），以“四血管阻断+体循环低血压”法制作兔全脑缺血再灌注损伤的模型。C组在再灌注时将bFGF 30μg/kg经耳缘静脉注入，此后以10μg.kg-1 .h-1微泵输入持续6h，对照组仅应用等剂量的生理盐水，假手术组光分离血管而不作其他处理。持续监测动脉血压、体温、心率，分别于夹闭血管前（base）、缺血30min（0h）及再灌注0.5、1、3、6h后测定血清肿瘤坏死因子α（TNF-α）、白细胞介素-1（IL-1）、白细胞介素-8（IL-8）的水平，实验结束取脑组织制作病理切片。 结果 3组动物基点的TNF-α、IL-1、IL-8值无明显差异，B、C两组缺血30min后均有所升高（以TNF-α明显），再灌注后进一步升高（与A组比较，P＜0.05；除外IL-8），再灌注6h时达峰值。相同时点TNF-α、IL-1值C组较B组为低，但部分时点差异没有显著性意义，IL-8值无差异。C组脑组织的镜下病理改变比B组轻。 结论 炎症反应介导脑的缺血再灌注损伤， 碱性成纤维细胞生长因子通过减少减少再灌注过程中炎症因子的生成及减轻它们介导的损伤，发挥脑保护作用。 【关键词】 碱性成纤维细胞生长因子；脑损伤/缺血再灌注；肿瘤坏死因子α；白细胞介素-1；白细胞介素-8 Effect of basic fibroblast growth factor on serum inflammatory factors in rabbits following cerebral ischemia-reperfusion injury MA Yue-feng, ZHANG Mao, JIANG Guan-yu, et al. Department of Emergency, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China 【Abstract】 Objective To observe the effect of basic fibroblast growth factor on serum inflammatory factors in rabbits following cerebral ischemia-reperfusion injury, and study its mechanism of neuroprotective effect. Methods Twenty-four rabbits were randomly divided into three groups. Cerebral ischemia-reperfusion injury was induced by obstruction of four vessels and systemic hypotension. bFGF was injected intravenously at the dose of 30μg/kg, then infused with 10μg.kg-1 .h-1 for 6 hours in bFGF group (Group C). The same volume of normal saline was applied in control group (Group B) and no advanced procedure was taken but dissection of four vessels in false-operation group (Group A). Blood pressure, heart rate and body temperature were continuously monitored. Blood samples were taken for determination of serum tumor necrosis factor-α(TNF-α), interleukin-1 (IL-1), interleukin-8 (IL-8) at the time of base, 0, 0.5, 1, 3, 6 h after reperfusion. Cerebral pathological features were observed. Results At the time of base, TNF-α、IL-1、IL-8 were not different among three groups. They were increased in group B and C after ischemia (obvious for TNF-α), then continuously rose and reached their peaks at 6h after reperfusion (compared with group A, P＜0.05; excluding IL-8＝). TNF-αand IL-1 in group C were lower than those in group B at the same time, but with P＞0.05 sometimes, and IL-8 was not different between the two groups. There were milder pathological changes in group C. Conclusions Inflammatory factors are enrolled in cerebral ischemia-reperfusion injury. bFGF has neuroprective effects by reducing production of inflammatory factors and antagonizing their adverse effect. 【Key words】 Basic fibroblast growth factor; Brain injury/Ischemia-reperfusion; Tumor necrosis factor-α; Interleukin-1; Interleukin-8